Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

Contact

Characterization of HIV-1 RNA forms in the plasma of patients undergoing successful HAART.

Abstract

An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 48 copies/ml. Sequences derived from plasma were compared to those from CD14+ CD16 +monocytes and CD4+ T cells. The plasma sequences were most closely related to those amplified from monocytes, suggesting that during successful antiretroviral therapy, the predominant plasma virus originates from myeloid cells. By characterizing HIV-1 RNA sequences from 8 ml of plasma while avoiding multiple steps, which can lead to contamination and deterioration, this method can help elucidate the viral forms in patients with therapeutically suppressed HIV-1. Understanding the source of residual viremia is crucial in developing approaches for viral eradication.

Authors: Lopez CA, Vazquez M, Hill MD, Del C Colon M, Porrata-Doria T, Johnston IC, Lorenzo E.
Journal: Arch Virol. 155: 895-903
Year: 2010
PubMed: Find in PubMed