Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

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Detection of human TNF-alpha mRNA by NASBA

Abstract

A method to amplify and detect TNF-alpha mRNA from primed Mono Mac 6 cells is described. A silica-based extraction system was utilised for preparation of cell extracts and specific oligonucleotide primers were designed for amplification of TNF-alpha mRNA by the NASBA process. Amplification products were detected using either a liquid hybridisation assay, with analysis by polyacrylamide gel electrophoresis, or a plate hybridisation system. The method has many potential applications for the study of inflammatory cytokines and cellular mRNAs in cell culture and clinical samples.

Authors: Darke, B.M., Jackson, S.K., Hanna, S.M., Fox, J.D.
Journal: J. Immunol. Meth. 212: 19-28
Year: 1998
PubMed: Find in PubMed